Growth of exocrine acinar cells on a reconstituted basement membrane gel
Identifieur interne : 001D36 ( Main/Exploration ); précédent : 001D35; suivant : 001D37Growth of exocrine acinar cells on a reconstituted basement membrane gel
Auteurs : Constance Oliver [États-Unis] ; Judith F. Waters [États-Unis] ; Carolyn L. Tolbert [États-Unis] ; Hynda K. Kleinman [États-Unis]Source :
- In Vitro Cellular & Developmental Biology [ 0883-8364 ] ; 1987-07-01.
English descriptors
- KwdEn :
- Teeft :
- Acinar, Acinar ceils, Acinar cells, Amylase, Basement membrane, Biol, Ceils, Cell biol, Cell division, Cell growth, Cell maintenance, Collagen, Confluency, Culture conditions, Decarboxylase, Electron microscopy, Epidermal, Epidermal growth factor, Epithelial, Epithelial cells, Exocrine, Exocrine acinar cells, Exorbital lacrimal gland, Extracellular matrix, Glutathione, Granule, Growth factors, Lacrimal, Laminin, Matrix, Membrane, Nutrient mixture, Ornithine, Ornithine decarboxylase activity, Pancreas, Pancreatic, Pancreatic acinar cells, Pancreatic acini, Parotid, Parotid acinar cells, Parotid gland, Peroxidase, Present study, Primaria, Primaria dishes, Protein synthesis, Reconstituted, Reconstituted basement membrane, Rinsed, Scanning electron microscopy, Secretory, Secretory granules, Substrata, Substratum, Sucrose, Sucrose buffer, Tissue culture dishes.
Abstract
Summary: Methods have been developed for culturing a dividing population of morphologically differentiated rat parotid, lacrimal, and pancreatic acinar cells in vitro. Isolated acinar cells were plated onto tissue culture dishes coated with a three-dimensional, reconstituted basement membrane gel. After attachment in Ham’s nutrient mixture F12, the cells were cultured at 35°C in F12 supplemented with 10% heat inactivated rat serum, epidermal growth factor, dexamethasone, insulin, transferrin, selenium, putrescine, reduced glutathione, ascorbate, penicillin, streptomycin, and the appropriate secretagogue. Under these conditions, the cells attached rapidly and DNA synthesis was initiated within 2 to 3 d. Although the cells flattened on the substratum, they continued to maintain their differentiated morphology. The cells contained secretory granules, and the secretory enzymes peroxidase and amylase could be detected. The use of a reconstituted basement membrane gel proved critical for the attachment and growth of exocrine acinar cells.
Url:
DOI: 10.1007/BF02628416
Affiliations:
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Waters</name><affiliation wicri:level="2"><country xml:lang="fr">États-Unis</country><placeName><region type="state">Maryland</region></placeName><wicri:cityArea>Laboratory of Oral Biology and Physiology, National Institute of Dental Research, National Institutes of Health, Bldg. 10, Room 1A23, 20892, Bethesda</wicri:cityArea></affiliation></author><author><name sortKey="Tolbert, Carolyn L" sort="Tolbert, Carolyn L" uniqKey="Tolbert C" first="Carolyn L." last="Tolbert">Carolyn L. Tolbert</name><affiliation wicri:level="2"><country xml:lang="fr">États-Unis</country><placeName><region type="state">Maryland</region></placeName><wicri:cityArea>Laboratory of Oral Biology and Physiology, National Institute of Dental Research, National Institutes of Health, Bldg. 10, Room 1A23, 20892, Bethesda</wicri:cityArea></affiliation></author><author><name sortKey="Kleinman, Hynda K" sort="Kleinman, Hynda K" uniqKey="Kleinman H" first="Hynda K." last="Kleinman">Hynda K. Kleinman</name><affiliation wicri:level="2"><country xml:lang="fr">États-Unis</country><placeName><region type="state">Maryland</region></placeName><wicri:cityArea>Laboratory of Developmental Biology and Anomalies (H. K. K.), National Institute of Dental Research, National Institutes of Health, Bldg. 10, Room 1A23, 20892, Bethesda</wicri:cityArea></affiliation></author></analytic><monogr></monogr><series><title level="j">In Vitro Cellular & Developmental Biology</title><title level="j" type="abbrev">In Vitro Cell Dev Biol</title><idno type="ISSN">0883-8364</idno><idno type="eISSN">1475-2689</idno><imprint><publisher>Springer-Verlag</publisher><pubPlace>New York</pubPlace><date type="published" when="1987-07-01">1987-07-01</date><biblScope unit="volume">23</biblScope><biblScope unit="issue">7</biblScope><biblScope unit="page" from="465">465</biblScope><biblScope unit="page" to="473">473</biblScope></imprint><idno type="ISSN">0883-8364</idno></series></biblStruct></sourceDesc><seriesStmt><idno type="ISSN">0883-8364</idno></seriesStmt></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>basement membrane</term><term>exorbital lacrimal gland</term><term>pancreas</term><term>parotid gland</term></keywords><keywords scheme="Teeft" xml:lang="en"><term>Acinar</term><term>Acinar ceils</term><term>Acinar cells</term><term>Amylase</term><term>Basement membrane</term><term>Biol</term><term>Ceils</term><term>Cell biol</term><term>Cell division</term><term>Cell growth</term><term>Cell maintenance</term><term>Collagen</term><term>Confluency</term><term>Culture conditions</term><term>Decarboxylase</term><term>Electron microscopy</term><term>Epidermal</term><term>Epidermal growth factor</term><term>Epithelial</term><term>Epithelial cells</term><term>Exocrine</term><term>Exocrine acinar cells</term><term>Exorbital lacrimal gland</term><term>Extracellular matrix</term><term>Glutathione</term><term>Granule</term><term>Growth factors</term><term>Lacrimal</term><term>Laminin</term><term>Matrix</term><term>Membrane</term><term>Nutrient mixture</term><term>Ornithine</term><term>Ornithine decarboxylase activity</term><term>Pancreas</term><term>Pancreatic</term><term>Pancreatic acinar cells</term><term>Pancreatic acini</term><term>Parotid</term><term>Parotid acinar cells</term><term>Parotid gland</term><term>Peroxidase</term><term>Present study</term><term>Primaria</term><term>Primaria dishes</term><term>Protein synthesis</term><term>Reconstituted</term><term>Reconstituted basement membrane</term><term>Rinsed</term><term>Scanning electron microscopy</term><term>Secretory</term><term>Secretory granules</term><term>Substrata</term><term>Substratum</term><term>Sucrose</term><term>Sucrose buffer</term><term>Tissue culture dishes</term></keywords></textClass><langUsage><language ident="en">en</language></langUsage></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Summary: Methods have been developed for culturing a dividing population of morphologically differentiated rat parotid, lacrimal, and pancreatic acinar cells in vitro. Isolated acinar cells were plated onto tissue culture dishes coated with a three-dimensional, reconstituted basement membrane gel. After attachment in Ham’s nutrient mixture F12, the cells were cultured at 35°C in F12 supplemented with 10% heat inactivated rat serum, epidermal growth factor, dexamethasone, insulin, transferrin, selenium, putrescine, reduced glutathione, ascorbate, penicillin, streptomycin, and the appropriate secretagogue. Under these conditions, the cells attached rapidly and DNA synthesis was initiated within 2 to 3 d. Although the cells flattened on the substratum, they continued to maintain their differentiated morphology. The cells contained secretory granules, and the secretory enzymes peroxidase and amylase could be detected. The use of a reconstituted basement membrane gel proved critical for the attachment and growth of exocrine acinar cells.</div></front></TEI><affiliations><list><country><li>États-Unis</li></country><region><li>Maryland</li></region></list><tree><country name="États-Unis"><region name="Maryland"><name sortKey="Oliver, Constance" sort="Oliver, Constance" uniqKey="Oliver C" first="Constance" last="Oliver">Constance Oliver</name></region><name sortKey="Kleinman, Hynda K" sort="Kleinman, Hynda K" uniqKey="Kleinman H" first="Hynda K." last="Kleinman">Hynda K. Kleinman</name><name sortKey="Tolbert, Carolyn L" sort="Tolbert, Carolyn L" uniqKey="Tolbert C" first="Carolyn L." last="Tolbert">Carolyn L. Tolbert</name><name sortKey="Waters, Judith F" sort="Waters, Judith F" uniqKey="Waters J" first="Judith F." last="Waters">Judith F. Waters</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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